简 历

个人信息

姓名：李爱涛

电话：18871879016

邮箱：aitaoli@hubu.edu.cn; aitaoli@163.com

湖北大学 教授，博导

省部共建生物催化与酶工程国家重点实验室副主任

教育背景

2017年10月～至今：湖北大学生命科学学院，教授

2014年～2017年：德国马普所（煤炭化学研究所），博士后研究员

2011年～2014年：新加坡国立大学化学与生物分子工程系，研究员

2005年～2010年：华东理工大学生物反应器工程国家重点实验室，博士学位

2001年～2005年：河北工业大学，生物工程系本科生，学士学位

学术兼职

《Bioresources and Bioprocessing》领衔客座编辑(leading guest editor)，《Green Synthesis and Catalysis》青年编委，《Frontiers in Bioengineering and Biotechnology》编委。湖北省生物催化与酶工程技术引智示范基地负责人，湖北省微生物学会理事，中国微生物学会会员。

研究方向

1. 酶定向进化方法学的开发（计算机辅助的精准设计、机器学习的酶分子改造）

2. 细胞色素P450酶学及酶工程（高效P450人工酶元件的创制与改造）

3. 细胞色素P450在功能化学品生物合成中的应用（化学品人工生物合成途径的创建）

科研项目

1. 中华人民共和国科学技术部，“合成生物学”重点研发计划课题项目，2019YFA0905002，人工途径生物酶元件的理性设计与结构改造，2020-1至2024-12，500万元，在研，主持

2. 国家自然科学基金委员会，面上项目，21977026，新型P450甾体羟化酶的分子调控机制及其理性改造，2020-01至 2023-12，66万元，在研，主持

3. 湖北省科技厅外专处，引智基地项目，XKCX2019000002，生物催化与酶工程技术， 2020-01至 2021-12，40万元，主持，在研

4. 国家自然科学基金委员会，青年科学基金项目，21702052，基于P450酶定向羟化的去甲（伪）麻黄碱对映体新合成途径研究，2018-01至 2020-12，26万元，在研，主持

5. P450氧化酶催化合成苯甲醇，保定保利瑞合生科技有限公司，20万，在研，主持

6. 生物反应器工程国家重点实验室开放课题：7β-甾体羟化酶分子改造及其催化机制研究，2018-08至2020-07，（一等资助，10万元），结题。

获奖及荣誉

1. 湖北省“楚天学子”，2017年

2. 湖北省“百人计划”，2018年

3. 湖北省“杰出青年基金”，2020年

4. 本科班优秀班主任，2019-2020年度

5. 优秀教师奖，2018-2020年度

6. 指导研究生获国家励志奖学金，2019年

7. 研究生获得国家留学基金委（CSC）资助，格罗宁根大学攻读博士学位，2020年

8. 指导学生获全国大学生生命科学创新创业大赛三等奖，2019年

代表性论文

2020年：

1. Xiaodong Zhang#, Yaqin Peng#, Jing Zhao#, Qian Li, Xiaojuan Yu, Carlos G Acevedo-Rocha, Ai-Tao Li.* Bacterial cytochrome P450-catalyzed regio-and stereoselective steroid hydroxylation enabled by directed evolution and rational design. Bioresources and Bioprocessing, 2020, 7 (1), 2

2. Ren-Jie Li, Zhongwei Zhang, Carlos G Acevedo-Rocha, Jing Zhao*, Ai-Tao Li.* Biosynthesis of organic molecules via artificial cascade reactions based on cytochrome P450 monooxygenases. Green Synthesis and Catalysis, 2020, Accepted.

3. Ai-Tao Li,* Carlos G Acevedo-Rocha, Lorenzo D’Amore, Jinfeng Chen, Yaqin Peng, Marc Garcia-Borràs, Chenghua Gao, Jinmei Zhu, Harry Rickerby, Sílvia Osuna*, Jiahai Zhou*, Manfred T Reetz*. Regio‐and Stereoselective Steroid Hydroxylation at the C7‐Position by Cytochrome P450 Monooxygenase Mutants. Angew. Chem. Int. Ed. 2020, 59, 12499–12505, (Hot paper)

4. Ge Qu, # Ai-Tao Li,# Carlos G Acevedo‐Rocha,# Zhoutong Sun*, Manfred T Reetz*. The crucial role of methodology development in directed evolution of selective enzymes. Angew. Chem. Int. Ed. 2020, 59, 13204–13231.

2019年：

5. Hangyu Zhou, Binju Wang, Fei Wang, Xiaojuan Yu, Lixin Ma, Ai-Tao Li*, Manfred T. Reetz*. Chemo- and Regioselective Dihydroxylation of Benzene to Hydroquinone Enabled by Engineered Cytochrome P450 Monooxygenase. Angew. Chem. Int. Ed. 2019, 58, 764–768, 杂志封面

6. Ai-Tao Li#, Ge Qu#, Zhoutong Sun*, Manfred T Reetz*. Statistical analysis of the benefits of focused saturation mutagenesis in directed evolution based on reduced amino acid alphabets. ACS Catalysis. 2019, 9, 7769-7778.

7. Guochao Xu, Cheng Zhu, Ai-Tao Li, Yan Ni, Ruizhi Han, Jieyu Zhou, Ye Ni*. Engineering an Alcohol Dehydrogenase for Balancing Kinetics in NADPH Regeneration with 1, 4-Butanediol as a Cosubstrate. ACS Sustainable Chemistry Engineering. 2019, 7, 15706-15714.

8. Ruyi He, Longyu Wang, Fei Wang, Wenqiang Li, Yang Liu, Ai-Tao Li, Yuan Wang, Wuxiang Mao, Chao Zhai*, Lixin Ma*. Pyrococcus furiosus Argonaute-mediated nucleic acid detection. Chemical Communications, 2019, 55, 13219-13222

9. Hangyu Zhou, Jing Zhao, Ai-Tao Li*, Manfred T Reetz*. Chemical and Biocatalytic Routes to Arbutin. Molecules, 2019, 24, 3303

2018年：

10. Ai-Tao Li, Carlos G. Acevedo-Rocha, Manfred T. Reetz. Boosting the Efficiency of Site-Saturation Mutagenesis for a Difficult-to-Randomize Gene by a Two-Step PCR Strategy. Applied Microbiology and Biotechnology. 2018, 102, 6095-6103.

11. Wenwen She, Jing Ni, Ke Shui, Fei Wang, Ruyi He, Jinhui Xue, Manfred T. Reetz, Aitao Li*, Lixin Ma*. Rapid and error-free site-directed mutagenesis by a PCR-free in vitro CRISPR/Cas9-mediated mutagenic system. ACS Synthetic Biology 2018, 7, 2236-2244, 杂志封面

12. Ai-Tao Li, Carlos G. Acevedo-Rocha, Zhoutong Sun, Tony Cox, Jia (Lucy) Xu, and Manfred T. Reetz*. Beating Bias in the Directed Evolution of Proteins: Combining High-Fidelity on-Chip Solid-Phase Gene Synthesis with Efficient Gene Assembly for Combinatorial Library Construction. ChemBioChem 2018，19 (3), 221-228 (被选为杂志封面).

13. Ai-Tao Li, zhoutong Sun, MT Reetz. Solid-Phase Gene Synthesis for Mutant Library Construction: The Future of Directed Evolution? ChemBioChem 2018, 19 (19), 2023-2032.

2017年：

14. Ai-Tao Li, Binju Wang, Adriana Ilie, Kshatresh D. Dubey, Gert Bange, Ivan V. Korendovych, Sason Shaik, Manfred T. Reetz. A redox-mediated Kemp eliminase. Nature Communications, 2017, 8, 14876.

15. Yucai He , Yun Ding , Cuiluan Ma , Junhua Di , Chunxia Jiang and Ai-Tao Li*. One-pot conversion of biomass-derived xylose to furfuralcohol by a chemo-enzymatic sequential acid-catalyzed dehydration and bioreduction. Green Chemistry, 2017, 19 (16), 3844-3850.

2017年之前：

16. Ai-Tao Li, Adriana Ilie, Zhoutong Sun, Richard Lonsdale, Jian-He Xu, Manfred T. Reetz. Whole-Cell-Catalyzed Multiple Regio- and Stereoselective Functionalizations in Cascade Reactions Enabled by Directed Evolution. Angewandte Chemie International Edition. 2016, 128,12205-12208.

17. Ai-Tao Li, Shuke Wu, Joseph P Adams, Radka Snajdrova, Zhi Li. Asymmetric Epoxidation of Alkene and Benzylic Hydroxylation with P450tol from Rhodococcus coprophilus TC-2. Chemical Communications, 2014, 50, 8771-8774. (higlighted in synfacts)

18. Ai-Tao Li, Ji Liu, Son Q. Pham, Zhi Li. Engineered P450pyr monooxygenase for asymmetric epoxidation of alkenes with unique and high enantioselectivity. Chemical Communications, 2013, 49, 11572-11574.

19. Ai-Tao Li, TPN Ngo, Jingyong Yan, Kaiyuan Tian, Zhi Li. Whole-cell based solvent-free system for one-pot production of biodiesel from waste grease. Bioresource Technology, 2012, 114: 725-729.

20. Ai-Tao Li, Hui-Lei Yu, Jiang Pan, Jian-Dong Zhang, Jian-He Xu, Guo-Qiang Lin. Resolution of racemic sulfoxides with high productivity and enantioselectivity by a Rhodococcus sp. strain as an alternative to biooxidation of prochiral sulfides for efficient production of enantiopure sulfoxides. Bioresource Technology, 2011, 102: 1537-1542.

21. Ai-Tao Li, Jian-Dong Zhang, Hui-Lei Yu, Jiang Pan, Jian-He Xu. Significantly improved asymmetric oxidation of sulfide with resting cells of Rhodococcus sp. in a biphasic system. Process Biochemistry, 2011, 46: 689-694.

22. Ai-Tao Li, Jian-He Xu, Guo-Qiang Lin. Isolation of Rhodococcus sp. ECU0066: A new sulfide monooxygenase producing strain for asymmetric sulfoxidation. Applied and Environmental Microbiology, 2009, 75: 551-556.

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